Predisposition for cholera of individuals with O blood group. Possible evolutionary significance. Lindesmith L. Human susceptibility and resistance to Norwalk virus infection. Role of apolipoprotein E4 in protecting children against early childhood diarrhea outcomes and implications for later development. Corthesy B. Roundtrip ticket for secretory IgA: role in mucosal homeostasis? Neutra M.
Mucosal vaccines: the promise and the challenge. Robbins J. Hypothesis for vaccine development: protective immunity to enteric diseases caused by nontyphoidal salmonellae and shigellae may be conferred by serum IgG antibodies to the O-specific polysaccharide of their lipopolysaccharides.
Sztein M. Cell-mediated immunity and antibody responses elicited by attenuated Salmonella enterica Serovar Typhi strains used as live oral vaccines in humans. Cytotoxic T lymphocytes after oral immunization with attenuated vaccine strains of Salmonella typhi in humans. Salerno-Goncalves R. Holmgren J. Mucosal immunity and vaccines.
Losonsky G. Systemic and mucosal immune responses to rhesus rotavirus vaccine MMU Czerkinsky C. Antibody-producing cells in peripheral blood and salivary glands after oral cholera vaccination of humans.
Kantele A. Antibody-secreting cells in the evaluation of the immunogenicity of an oral vaccine. Different profiles of the human immune response to primary and secondary immunization with an oral Salmonella typhi Ty21a vaccine. Secondary Vibrio cholerae-specific cellular antibody responses following wild-type homologous challenge in people vaccinated with CVD HgR live oral cholera vaccine: changes with time and lack of correlation with protection. B cell responses in gastric antrum and duodenum following oral inactivated Helicobacter pylori whole cell HWC vaccine and LT RG in H pylori seronegative individuals.
Unique characteristics of the intestinal immune system as an inductive site after antigen reencounter. Kunkel E. Plasma-cell homing.
Brandtzaeg P. Mucosal B cells: phenotypic characteristics, transcriptional regulation, and homing properties. Entamoeba histolytica infection in children and protection from subsequent amebiasis. Coulson B. Role of coproantibody in clinical protection of children during reinfection with rotavirus.
Fordtran J. Speculations on the pathogenesis of diarrhea. Binder, H. Electrolyte transport in the mammalian colon. In Physiology of the gastrointestinal tract. Johnson, editor. Raven Press. Field M. Effect of cholera enterotoxin on ion transport across isolated ileal mucosa. Intestinal adenyl-cyclase activity in canine cholera: correlation with fluid accumulation. Galan J. Salmonella interactions with host cells: type III secretion at work. Cell Dev. Moss J. Mechanism of action of choleragen and E.
Mezoff A. Escherichia coli enterotoxin STa binds to receptors, stimulates guanyl cyclase, and impairs absorption in rat colon. Hughes J. Role of cyclic GMP in the action of heat-stable enterotoxin of Escherichia coli. Cassuto J. On the role of intramural nerves in the pathogenesis of cholera toxin-induced intestinal secretion. Jodal M. Nerve involvement in fluid transport in the inflamed rat jejunum.
Castagliuolo I. Neuronal involvement in the intestinal effects of Clostridium difficile toxin A and Vibrio cholerae enterotoxin in rat ileum. Ball J. Rotavirus NSP4: a multifunctional viral enterotoxin. Viral Immunol. Beau I. An NSP4-dependant mechanism by which rotavirus impairs lactase enzymatic activity in brush border of human enterocyte-like Caco-2 cells. Cell Microbiol. Sansonetti P. Debugging how bacteria manipulate the immune response.
Role of interleukin-1 in the pathogenesis of experimental shigellosis. Hecht G. Microbes and microbial toxins: paradigms for microbial-mucosal interactions. Enteropathogenic Escherichia coli: physiological alterations from an extracellular position. Liver Physiol. Troeger H. Diarrheal mechanisms of human norovirus infection. Gill R. Logan C. Real-time reverse transcription PCR detection of norovirus, sapovirus and astrovirus as causative agents of acute viral gastroenteritis.
Samie A. Prevalence of Campylobacter species, Helicobacter pylori and Arcobacter species in stool samples from the Venda region, Limpopo, South Africa: studies using molecular diagnostic methods. Detection of diarrhoea-causing protozoa in general practice patients in The Netherlands by multiplex real-time PCR. Enteroaggregative Escherichia coli in Venda, South Africa: distribution of virulence-related genes by multiplex polymerase chain reaction in stool samples of human immunodeficiency virus HIV -positive and HIV-negative individuals and primary school children.
Nataro J. Ochoa T. Effect of lactoferrin on enteroaggregative E. Cell Biol. Measurement of fecal lactoferrin as a marker of fecal leukocytes. J Clin. Greenberg D. Markers of inflammation in bacterial diarrhea among travelers, with a focus on enteroaggregative Escherichia coli pathogenicity. Kane S. Fecal lactoferrin is a sensitive and specific marker in identifying intestinal inflammation.
Venkataraman S. Faecal lactoferrin as a predictor of positive faecal culture in south Indian children with acute diarrhoea. Calprotectin and lactoferrin in the assessment of intestinal inflammation and organic disease. Colorectal Dis. Scarpa M. Colon Rectum. Amati L. New insights into the biological and clinical significance of fecal calprotectin in inflammatory bowel disease.
Lunn P. The impact of infection and nutrition on gut function and growth in childhood. Haeney M. Evaluation of xylose absorption as measured in blood and urine: a one-hour blood xylose screening test in malabsorption.
Intestinal permeability, mucosal injury, and growth faltering in Gambian infants. Calain P. Can oral cholera vaccination play a role in controlling a cholera outbreak? Lucas M. Effectiveness of mass oral cholera vaccination in Beira, Mozambique. Murphy T. Intussusception among infants given an oral rotavirus vaccine. Ruiz-Palacios G. Safety and efficacy of an attenuated vaccine against severe rotavirus gastroenteritis.
Vesikari T. Safety and efficacy of a pentavalent human-bovine WC3 reassortant rotavirus vaccine. Lin F. The efficacy of a Salmonella typhi Vi conjugate vaccine in two-to-five-year-old children. Mai N. Persistent efficacy of Vi conjugate vaccine against typhoid fever in young children. Evaluation of Salmonella enterica serovar Typhi Ty2 aroC-ssaV- M01ZH09, with a defined mutation in the Salmonella pathogenicity island 2, as a live, oral typhoid vaccine in human volunteers.
Tacket C. Immune responses to an oral typhoid vaccine strain that is modified to constitutively express Vi capsular polysaccharide. Hohmann E. Qadri F. Peru, a live attenuated oral cholera vaccine, is safe and immunogenic in Bangladeshi toddlers and infants.
Garcia L. The vaccine candidate Vibrio cholerae is protective against cholera in healthy volunteers. Orr N. Community-based safety, immunogenicity, and transmissibility study of the Shigella sonnei WRSS1 vaccine in Israeli volunteers. Katz D. Two studies evaluating the safety and immunogenicity of a live, attenuated Shigella flexneri 2a vaccine SC and excretion of vaccine organisms in North American volunteers.
Levine M. Clinical trials of Shigella vaccines: two steps forward and one step back on a long, hard road. Cohen D. Double-blind vaccine-controlled randomised efficacy trial of an investigational Shigella sonnei conjugate vaccine in young adults. Oaks E. Development and evaluation of a Shigella flexneri 2a and S. Fries L. Safety and immunogenicity of a proteosome-Shigella flexneri 2a lipopolysaccharide vaccine administered intranasally to healthy adults.
McKenzie R. Safety and immunogenicity of an oral, inactivated, whole-cell vaccine for Shigella sonnei: preclinical studies and a Phase I trial.
Core-linked LPS expression of Shigella dysenteriae serotype 1 O-antigen in live Salmonella Typhi vaccine vector Ty21a: preclinical evidence of immunogenicity and protection. Savarino, S. Efficacy of an oral inactivated whole-cell enterotoxigenic E. June 22— Arlington, Virginia, USA. National Foundation for Infectious Diseases. Daley A. Genetically modified enterotoxigenic Escherichia coli vaccines induce mucosal immune responses without inflammation.
Barry E. Transcutaneous immunization with the heat-labile toxin LT of enterotoxigenic Escherichia coli ETEC : protective efficacy in a double-blind, placebo-controlled challenge study.
Humoral, mucosal, and cellular immune responses to oral Norwalk virus-like particles in volunteers. Scott D. Vaccines against Campylobacter jejuni. Sougioultzis S. Clostridium difficile toxoid vaccine in recurrent C. Boullier S. Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge. Microbes Infect. Houpt E. Jenkins M. Present and future control of cryptosporidiosis in humans and animals.
Expert Rev. The humoral and cellular immune responses in mice induced by DNA vaccine expressing the sporozoite surface protein of Cryptosporidium parvum. DNA Cell Biol. Konadu E. Phase 1 and phase 2 studies of Salmonella enterica serovar paratyphi A O-specific polysaccharide-tetanus toxoid conjugates in adults, teenagers, and 2- to 4-year-old children in Vietnam.
Thillainayagam A. Enhancing clinical efficacy of oral rehydration therapy: is low osmolality the key? Rao M. Oral rehydration therapy: new explanations for an old remedy. Binder H. Characterization of butyrate-dependent electroneutral Na-Cl absorption in the rat distal colon. Pflugers Arch. Raghupathy P. Amylase-resistant starch as adjunct to oral rehydration therapy in children with diarrhea.
J Pediatr. Ramakrishna B. Amylase-resistant starch plus oral rehydration solution for cholera. A randomized controlled trial of glucose versus amylase resistant starch in hypo-osmolar oral rehydration solution for adult acute dehydrating diarrhea.
PLos ONE. Sazawal S. Zinc supplementation in young children with acute diarrhea in India. Bhatnagar S. Zinc with oral rehydration therapy reduces stool output and duration of diarrhea in hospitalized children: a randomized controlled trial.
Hoque K. Zinc in the treatment of acute diarrhea: current status and assessment. DeSesso J. Anatomical and physiological parameters affecting gastrointestinal absorption in humans and rats. Food Chem. Vanderhoof J. Enteral and parenteral nutrition in the care of patients with short-bowel syndrome. Lima A. Cotransport of sodium with glutamine, alanine and glucose in the isolated rabbit ileal mucosa.
Blikslager A. Glutamine transporter in crypts compensates for loss of villus absorption in bovine cryptosporidiosis. Lima N. Wasting and intestinal barrier function in children taking alanyl-glutamine-supplemented enteral formula.
Bushen O. Diarrhea and reduced levels of antiretroviral drugs: improvement with glutamine or alanyl-glutamine in a randomized controlled trial in northeast Brazil. Infect Dis. Carneiro-Filho B. Glutamine analogues as adjunctive therapy for infectious diarrhea. The effect of L-glutamine on salt and water absorption: a jejunal perfusion study in cholera in humans. Ribeiro J. Janes, PI , and R. Our research project will help fill this gap. Impacts Vibrio vulnificus V. The rapid detection of V.
The purpose of this study was to develop a rapid, user friendly and compact screening dipstick device utilizing V. The dipstick test strips were prepared by conjugating the species-specific anti V. The control line was prepared by using Goat anti Mouse IgG and sprayed on the membrane at the rate of 1. Specificity of the dipstick device was tested against pure culture of 8 vibrio strains while sensitivity was tested by utilizing serially diluted overnight grown V.
One ml from each sample was collect every hr for 6 hr and immediately tested with the dipstick. Our dipstick device successfully identified V. The lowest concentration of V. Our dipstick assay could be an answer to seafood industries rapid pathogen detection needs. Publications Qureshi, M. Janes and D. Journal of Applied Polymer Science Cole, M. Li, B. El-Zahab, M. Janes, D. Hayes, and I. Jadeja, R. Janes, and J. Dipstick assay for Vibrio vulnifus. Abstract P In addition, one refereed journal article was generated.
Janes was the lead PI on the project and assisted with the design of the research project, supervision of the student, and analysis of the data. Impacts Oysters from the estuarine environments are well known to be carriers of Vibrio species. Due to the foodborne illnesses caused by consuming raw oysters, the FDA may require all raw oysters to have a post-harvest treatment to reduce Vibrio species.
The current study was conducted to development a post-harvest treatment to reduce the levels of Vibrio vulnificus and Vibrio parahaemolyticus in shucked oysters by using low temperature treatments.
Shucked oysters g were collected in sterile Whirl-Pak bags and spiked with V. Inoculated oyster meat samples were placed in water baths for 12 min at four different temperatures 40, 45, 50 or 55degreeC.
After heating, oyster samples were rapidly cooled by placing the oyster meat on ice and immediately storing at 4degreeC. The following samples were analyzed for bacterial counts for all the four temperature treatments, inoculated oyster samples or control, heat-treated inoculated oysters without cooling, inoculated oysters heated then cooled on ice. The most effective treatment was 55degreeC which reduced the bacterial counts for both Vibrio species to non-detectable on day 0, 1 and 2.
In addition, the statistical analyses that we performed could not establish a correlation between HAdV detection and seasonal changes. However, the low incidence of these RNA viruses in sediment samples Other studies exploring HAV incidence in Brazil have also shown a low incidence in environmental samples Rigotto et al. The high incidence of these viruses in water samples collected from the collection points along the Sangradouro River indicate that these viruses may play an important role in the contamination of Peri Lagoon and alterations in this ecosystem.
RVA showed a marked winter seasonal peak In the present study, HAV displayed a higher incidence during summer Another study performed in Brazil by Rigotto et al.
Additionally, a study performed in Rio de Janeiro by Villar et al. However, it is important to consider the fact that HAV infections are underreported, and many cases are asymptomatic. None of the physicochemical parameters or sediments showed sufficiently consistent significant correlations with the presence of viruses or with HAdV infectivity. The lack of correlation between physicochemical and virological parameters has been well documented by other studies Lee et al.
This study also highlighted the importance of evaluating the ecosystems interconnected with the Sangradouro River to enhance the safety of drinking water and recreational water sources in this environment. This water may otherwise pose a threat to the population that is mainly responsible for the degradation of its quality in this lagoon and neighboring waters.
Impact Factor 1. CiteScore 2. This paper is Open Access via a Subscribe to Open model. Individuals can help sustain this model by contributing the cost of what would have been author fees.
Find out more here. Sign In or Create an Account. Advanced Search. Sign In. Skip Nav Destination Article Navigation. Close mobile search navigation Article navigation. Volume 14, Issue 1. Previous Article Next Article. Article Navigation. Research Article August 22 Elmahdy ; E.
This Site. Google Scholar. Fongaro ; G. Schissi ; C. Petrucio ; M. Barardi C. E-mail: celia. J Water Health 14 1 : — Article history Received:. Cite Icon Cite. This study was conducted at Peri Lagoon and the Sangradouro River.
Figure 1. View large Download slide. Primers and probes. Final conc. View Large. Point 1 Sum. Point 2 Sum. Point 3 Sum. Point 4 Sum. Point 5 Sum. Point 6 Sum. Surface water Sample temp. For the surface waters collected during summer, the incidence of HAdV was All 17 positive samples were found to have undamaged particles after nuclease treatment at concentrations ranging from 1.
For the winter collections, the HAdV incidence was Of these positive samples, No infectious units could be measured by PFU during the winter collections. Figure 2. Figure 3. The RVA incidence in the surface water samples collected during summer was In winter, this incidence was For the sediment samples collected during summer, the RVA incidence was 8.
Figure 4. Points of collection. Point 1 Wat. As presented in Figure 5 , the overall mean percentages for the seasonal occurrence of the three viruses showed that RVA was more frequently detected in both surface water and sediment during the winter season.
In contrast, the frequency of HAV detected in the summer samples was higher than that in the winter samples, but only for surface water. HAdV was the most prevalent virus and showed no difference in seasonal prevalence. Figure 5. Comparation of methods for concentrating human adenovirus, polyomavirus JC and norovirus in source waters and drinking water using quantitative PCR.
Search ADS. Faecal indicator bacteria are abundant in wet sand at freshwater beaches. Occurrence of adenovirus and other enteric viruses in limited contact freshwater recreational areas and bathing waters.
The inactivation of hepatitis A virus and other model viruses by UV irradiation. New tools for the study and direct surveillance of viral pathogens in water.
Detection and quantitation of infectious human adenoviruses and JC polyomaviruses in water by immunofluorescence assay.
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